{"created":"2023-06-19T06:32:38.681690+00:00","id":36,"links":{},"metadata":{"_buckets":{"deposit":"fbaef81b-8212-48cf-9eb3-091fe31de169"},"_deposit":{"created_by":5,"id":"36","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"36"},"status":"published"},"_oai":{"id":"oai:nupals.repo.nii.ac.jp:00000036","sets":[]},"author_link":["32"],"item_10010_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"The effect of random-mutagenesis in the killer toxin HM-1 on fungicidal activity"}]},"item_10010_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"キラートキシンは他の酵母の生育を阻害する活性（キラー活性）をもつ、酵母が生産するタンパク質の総称である。これまでに数種のキラートキシンの性質が明らかにされている。そのうち酵母Williopsis saturnus var. mrakiiが生産するキラートキシンHM-1は安定性が高く、Saccharomyces cerevisiae、Hansenula anomala などの酵母には強い抗真菌活性を示す。しかし、Candida albicans、Cryptococcus属などには活性を示さないため、医療への応用、実用化には至っていない。HM-1の活性を活かし医療への応用、実用化につなげるために本研究ではHM-1の遺伝子の一部をランダムに変異させ、その活性の変化を比較した。 \n今回分析したランダム突然変異導入クローン中にはキラー活性が上昇したものはなく、キラー活性が見られなかった。この結果から、本研究により変異させたアミノ酸がHM-1のキラー活性に重要であることが確認できた。ランダム変異を同時に複数のアミノ酸に導入することによってキラー活性を上げることは容易ではないのかもしれない。","subitem_description_type":"Abstract"}]},"item_10010_relation_18":{"attribute_name":"他の資源との関係","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"URI:  http://hdl.handle.net/10801/364 "}]}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山﨑, 道穂"}],"nameIdentifiers":[{"nameIdentifier":"32","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-24"}],"displaytype":"detail","filename":"06P190_山﨑  道穂.pdf","filesize":[{"value":"468.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"06P190_山﨑  道穂","url":"https://nupals.repo.nii.ac.jp/record/36/files/06P190_山﨑  道穂.pdf"},"version_id":"6304736f-1973-44b7-9ecb-5976294240e6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"酵母","subitem_subject_scheme":"Other"},{"subitem_subject":"キラートキシン","subitem_subject_scheme":"Other"},{"subitem_subject":"キラー活性","subitem_subject_scheme":"Other"},{"subitem_subject":"HM-1","subitem_subject_scheme":"Other"},{"subitem_subject":"ランダム突然変異","subitem_subject_scheme":"Other"},{"subitem_subject":"形質転換","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA配列分析","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"other","resourceuri":"http://purl.org/coar/resource_type/c_1843"}]},"item_title":"ランダム突然変異導入によるキラートキシンHM-1活性への影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ランダム突然変異導入によるキラートキシンHM-1活性への影響"}]},"item_type_id":"10010","owner":"5","path":["6"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-11-12"},"publish_date":"2012-11-12","publish_status":"0","recid":"36","relation_version_is_last":true,"title":["ランダム突然変異導入によるキラートキシンHM-1活性への影響"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2023-06-19T06:50:10.602875+00:00"}